Bacterial Genome Sizes Determined by DNA Renaturation Studies
نویسندگان
چکیده
منابع مشابه
Bacterial genome sizes determined by DNA renaturation studies.
The genome size of bacterial DNA is known only for a very few species. Autoradiographic studies by Cairns (1963) have shown that the DNA in Escherichia coli is organized into a single circular molecule, 1100 to 1400,um. long. Haemophilus infIuenzae was found by electron microscopy to contain a total length of DNA of about 800,um. (MacHattie, Berns & Thomas, 1965), corresponding to the DNA conte...
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The flow cytometry method was used to estimate the genome sizes of nine agriculturally important insects, including two coleopterans, five Hemipterans, and two hymenopterans. Among which, the coleopteran Lissorhoptrus oryzophilus (Kuschel) had the largest genome of 981 Mb. The average genome size was 504 Mb, suggesting that insects have a moderate-size genome. Compared with the insects in other...
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The rate of renaturation of fully denatured DNA is kinetically a second-order reaction. The reaction rate increases as the temperature decreases below T,t, reaching a broad flat maximum from 15 to 30°C below T, and then decreases with a further decrease in temperature. Let N be the complexity of the DNA or the number of base-pairs iu non-repeating sequences per virus or cell for the given DNA, ...
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ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1970
ISSN: 0022-1287
DOI: 10.1099/00221287-64-3-377